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Assay of glutamine synthetase in cerebrospinal fluid as a specific marker in Alzheimer's disease

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dc.creator Oettle, Nicola
dc.date 2012-09-17T10:36:29Z
dc.date 2012-09-17T10:36:29Z
dc.date 1997
dc.date.accessioned 2017-05-10T10:21:25Z
dc.date.available 2017-05-10T10:21:25Z
dc.identifier http://hdl.handle.net/11189/406
dc.identifier.uri http://hdl.handle.net/11189/406
dc.description Thesis submitted in fulfilment of the requirements for the Master's Degree in Medical Technology in the School of Life Sciences at the Cape Technikon
dc.description There is, at present, no recognised diagnostic biochemical marker of Alzheimer's Disease (AD). Recently, Gunnerson and Haley, (1992), reported that the presence of glutamine synthetase (GS) in cerebrospinal fluid (CSF) samples showed a 97% correlation with patients diagnosed as having AD. GS was detected by photolabelling with [y32P]2-azido-ATP or [y32P]8azido- ATP and visualisation following sodium dodecyl sulphate polyacrylamide gel electrophoresis (SOS-PAGE) and autoradiography. This study set out to reproduce Gunnerson and Haley's methodology for labelling sheep GS in CSF using [y32P]8-azidoATP, to develop this assay or possibly another, using a fluorescent probe of ATP binding sites, into a robust procedure suitable for a routine diagnostic laboratory, and finally to assess whether the presence of GS in CSF is indeed a marker of AD.
dc.language en
dc.publisher Cape Technikon
dc.rights http://creativecommons.org/licenses/by-nc-sa/3.0/za/
dc.subject Alzheimer's disease
dc.subject Cerebrospinal fluid -- Examination
dc.subject Glutamine synthetase
dc.title Assay of glutamine synthetase in cerebrospinal fluid as a specific marker in Alzheimer's disease
dc.type Thesis


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