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A study of entomopathogenic nematodes in the Greater Johannesburg region

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dc.contributor.advisor Gray VM en
dc.contributor.author Taylor SH en
dc.date.accessioned 2016-09-22T11:18:11Z
dc.date.available 2016-09-22T11:18:11Z
dc.date.created 2000 en
dc.date.submitted 2002 en
dc.identifier.uri http://hdl.handle.net/20.500.11892/109492
dc.description.abstract Entomopathogenic nematodes are of scientific importance because of their potential to be used as biological control agents of a wide variety of insect pests. Until now South African soils have not been extensively or systematically studied in terms of entomopathogenic nematodes. The aim of this research was to isolate and identify strains of entomopathogenic nematodes indigenous to South Africa. Fifty soil samples were collected from around Southern Africa, initially concentrating in the greater Johannesburg region. Eleven nematode isolates were obtained. Of these, only two isolates were successfully cultured in the laboratory. These two isolates were confirmed to be entomopathogenic. The genus and species of the isolates could not be established using morphological characteristics. The bacterial symbionts of the two isolates were isolated and a region of the 16S rRNA gene was sequenced. The sequences could not be matched with any published sequences of entomopathogenic nematode bacterial symbionts. This study confirms the presence of entomopathogenic nematodes in the greater Johannesburg region and other regions in South Africa. en
dc.language English en
dc.subject Zoology en
dc.subject Invertebrates: Platyhelminthes, Annelida, Nematoda en
dc.title A study of entomopathogenic nematodes in the Greater Johannesburg region en
dc.type Masters degree en
dc.description.degree MSc en

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