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Dye/antibody complexes for immunodiagnosis

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dc.contributor.author Ahmed N en
dc.date.accessioned 2016-09-22T11:18:14Z
dc.date.available 2016-09-22T11:18:14Z
dc.date.submitted 1998 en
dc.identifier.uri http://hdl.handle.net/20.500.11892/109541
dc.description.abstract The development of a cheap, visual antibody based diagnostic system, using colloidal and disperse dyes, was investigated as an alternative to the use of radioactivity or enzymes. Dye was bound to chicken antibodies(IgY) raised against sheep IgG. The conditions for the interaction of dye with antibody were optimised. The use of a 5mM phosphate buffered saline at pH 7.0 for the incubation of the dye and antibody complex, for a period of up to 120 minutes on ice was optimum. Dialysis studies showed that dye remained bound to the antibody, while the ELISA test showed that the presence of the dye on the antibody, had a minimal effect on the ability of the antibody to bind to the antigen. The dye/antibody complex was then used to identify antigens bound to nitrocellulose in both immunoblotting and western blotting studies. It was shown that a 1000 times dilution of the antigen could be detected visually when using the dye/antibody complex in immunoblotting studies, while the use of the complex for western blotting showed that this system could detect the antigen after the western transfer. The detection was specific since the complex did not bind to any non specific antigen used. en
dc.language English en
dc.title Dye/antibody complexes for immunodiagnosis en
dc.type Masters degree en
dc.description.degree MSc en

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