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The role of stress proteins HSP 70 and HSP 90 in cerebral malaria

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dc.contributor.author Rathupetsane PJ en
dc.date.accessioned 2016-09-22T11:18:14Z
dc.date.available 2016-09-22T11:18:14Z
dc.date.submitted 1997 en
dc.identifier.uri http://hdl.handle.net/20.500.11892/109544
dc.description.abstract Malarial parasites alter the host immune response by inducing an upregulation of cytoadherence receptors and increasing the secretion of pyrogenic cytokines. In order to prevent damage induced by inflammatory responses, cells express "stress" (heat shock) proteins which are known to inhibit secondary effects of tumour necrosis factor including the secretion of phospholipase A<sub>2</sub>.The function of these proteins appear to have been compromised in cerebral malaria patients, where elevated levels of both tumour necrosis factor and phospholipase A<sub>2</sub> are found. Examination of the expression of heat shock proteins during malaria infection may provide an insight into the pathophysiology bf malaria. <BR><BR> Monocytes were isolated from human blood by ficoll density centrifugation and maintained at optimal growth conditions. Monocytes were treated with different agents either in the presence or absence of malarial culture supernatant and thereafter subjected to heat shock. Monocytic lysate proteins were subjected to SDS-PAGE and western transfer. Monocyte expression of heat shock proteins was detected by using the enhanced chemiluminescence detection system. Toxicity assays for all treatments revealed a more than 80% viability of monocytes. A 90Kda band was not detected in control monocytes and monocytes exposed to malarial culture supernatant. A 90Kda band was detected in monocytes in all the other lanes. The anti-hsp 90 monoclonal antibody detected hsp 90 and degradation products. The anti-hsp 70 monoclonal antibody did not detect hsp 70. We confirmed with experiments with purified hsp 70 that the anti-hsp 70 monoclonal antibody is specific for hsp 70 implying that hsp 70 was not expressed in monocytes we were studying.The results were inconclusive concerning the effect of malarial antigens on monocyte heat shock response. The monocyte hsp 70 expression should be investigated further to elucidate the role of hsp 70 during malaria infection. en
dc.language English en
dc.title The role of stress proteins HSP 70 and HSP 90 in cerebral malaria en
dc.type Masters degree en
dc.description.degree MSc en

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