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Production of peroxidase by horseradish hairy root culture in a bioreactor

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dc.contributor.author Duneas N en
dc.date.accessioned 2016-09-22T11:18:14Z
dc.date.available 2016-09-22T11:18:14Z
dc.date.submitted 1993 en
dc.identifier.uri http://hdl.handle.net/20.500.11892/109550
dc.description.abstract Transformed roots of Armoracia rusticana (horseradish), induced by the phytopathogenic soil bacterium Agrobacterium rhizogenes, were cultured in a two litre bubble column reactor with the purpose of characterising their growth and production of horseradish peroxidase. Published methods were used to induce and transformed roots from horseradish. The low frequency of transformed root induction obtained when using published methods was attributed to overinoculation of explant tissues with A. rhizogenes. Transformed roots were however easily induced by a leaf disk method which was modified with the inclusion of an antibiotic purge procedure to assist in the killing of contaminating A. rhizogenes.<br><br> In axenic and non-axenic shaken flask culture, transformed roots were characterised by their numerous lateral branches and rapid growth in phytohormone-free growth media.<br><br> In the case of the bioreaction, the process was not able to reach completion due to fungal contamination which occurred at the very early stages of transformed root exponential growth phase (tenth day) . In the period the bioreaction proceeded (14 days), the transformed roots had accumulated a tenfold increase in biomass, secreted 13,2 enzyme units per litre and produced 340 enzyme units per gram transformed root.<br><br> A mathematical model which describes the bioreaction process in terms of carbon, nitrogen and mineral ion uptake and the resultant biomass accumulation with time was developed. en
dc.language English en
dc.title Production of peroxidase by horseradish hairy root culture in a bioreactor en
dc.type Masters degree en
dc.description.degree MSc en

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