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Tissue culture and cryopreservation of Daucus carota L

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dc.contributor.author Kiralfy S en
dc.date.accessioned 2016-09-22T11:18:14Z
dc.date.available 2016-09-22T11:18:14Z
dc.date.submitted 1995 en
dc.identifier.uri http://hdl.handle.net/20.500.11892/109553
dc.description.abstract Cryopreservation offers a viable and safe storage method for germplasm in contrast to more traditional germplasm conservation methods, but can be technically difficult and can involve the use of expensive equipment. The present study achieved the production and maintenance of callus and suspension cultures and somatic embryos of Daucus Carota. L. for use in cryopreservation experimentation. The effects of dehydration and the use of the cryoprotectant glycerol on the cryostorability of carrot callus using an ultra-fast freeze technique was also explored. Successful cryopreservation was achieved using this technique without dehydration or cryoprotection of the callus material, but with a relatively low efficiency of 23%. Dehydrating callus material improved cryopreservation efficiency to 38%, but the highest efficiency (65%) was observed when callus was dehydrated in conjunction with cryoprotection using a glycerol solution. en
dc.language English en
dc.title Tissue culture and cryopreservation of Daucus carota L en
dc.type Masters degree en
dc.description.degree MSc en


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