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Die ontwikkeling van elektrosproei-massaspektrometrie vir die bepaling van karnitienpalmitoieltransferase I aktiwiteit in fibroblaste en leukosiete

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dc.contributor.advisor Van der Westhuizen FH, Mr en
dc.contributor.advisor Pretorius PJ, Prof en
dc.contributor.author Nolte L en
dc.date.accessioned 2016-09-22T07:17:28Z
dc.date.available 2016-09-22T07:17:28Z
dc.date.created 1996 en
dc.date.submitted 1997 en
dc.identifier.uri http://hdl.handle.net/20.500.11892/11378
dc.description.abstract Carnitine Palmitoyltransferase I (CPT I) is one of the important enzymes associated with normal mitochondrial membrane transport of certain metabolites. The importance of the enzyme in normal energy production is well illustrated during fasting conditions when a large flux of long chain fatty acids must be transported over the mitochondrial membrane to undergo β-oxidation. Up to now CPT I activity has been assayed in various tissues, including liver, leukocytes, platelets and fibroblasts by the use of an isotope exchange forward assay which measures the rate of palmitoyl-L-[methyl-3H]carnitine formation from palmitoyl-CoA and L-[methyl-3H] carnitine. We have developed an electrospray ionization mass spectrometric method for detecting palmitoylcarnitine formation from palmitoyl-CoA and carnitine, thus avoiding the use of radio labelled isotopes. In this assay, time dependent conversion of free carnitine by CPT I to palmitoylcarnitine is measured quantitatively, relative to isotopically labelled palmitoylcarnitine, by parent ion monitoring of fragment ion m/z 85. The combination of high sensitivity and selectively of tandem mass spectrometry along with the environment friendly nature of the method makes it an ideal technique to measure CPT I activity. en
dc.language Afrikaans en
dc.subject Biochemistry en
dc.title Die ontwikkeling van elektrosproei-massaspektrometrie vir die bepaling van karnitienpalmitoieltransferase I aktiwiteit in fibroblaste en leukosiete en
dc.type Masters degree en
dc.description.degree MSc en


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