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Karakterisering van bakteriese ekstrasellul^ere ensieme

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dc.contributor.advisor Pretorius PJ, Prof en
dc.contributor.author Van Rooyen T en
dc.date.accessioned 2016-09-22T07:17:28Z
dc.date.available 2016-09-22T07:17:28Z
dc.date.created 1997 en
dc.date.submitted 1998 en
dc.identifier.uri http://hdl.handle.net/20.500.11892/11381
dc.description.abstract The continuously growing poultry industry has to combat the problem of waste in the form of dead and rejected animals and consequently the large amount of feathers. Feathers and feathermeal can be utilised as a protein food supplement however, the digestibility of feathermeal is poor, but can be improved by digestion with bacterial keratinases. Eight keratinolytic bacteria were isolated from different keratincontaining sources such as chickenfeathers, sheepwool and a leather factory. Three species were identified: "Bacillus anthracis, B. cereus" and "Alcailgenes faecalls". Optimal growth conditions for enzyme production were established and varied from 28 Celsius - 37 Celsius and the pH from 6 - 8. The keratinase enzymes of three of the isolates were partially purified. The optimum activity of these partially purifies enzymes was at a pH of 8 and a temperature range of 28 Celsius - 37 Celsius. The respective molecular weights of the enzymes obtained from "B. cereus" J and "A. faecalis" is about 45 kDa and that of "B. cereus" O about of 68kDa. "The results of parts of this study were presented at the following scientific meetings": T. van Rooyen, T. Reinecke & P.J. Pretorius "Isolering en karakterisering van keratinolitiese ensieme uit bakterie�". Kongres of the Division Biology of the South-African Academy for Science and Arts. PU vir CHO, 1997 (Poster) T. van Rooyen, T. Reinecke & P.J. Pretorius "Isolation and characterisation of keratinolytic enzymes from bacteria". Regionalsimposium of the South-African Society for Biochemistry and Molecular Biology. Bloemfontein, 1997(Recital). en
dc.language Afrikaans en
dc.subject Biochemistry en
dc.title Karakterisering van bakteriese ekstrasellul^ere ensieme en
dc.type Masters degree en
dc.description.degree MSc en

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