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Biodegradering van sintetiese nitro-aromatiese verbindings deur mikro-organismes

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dc.contributor.author Janse van Rensburg PJ en
dc.date.accessioned 2016-09-22T07:17:31Z
dc.date.available 2016-09-22T07:17:31Z
dc.date.submitted 1997 en
dc.identifier.uri http://hdl.handle.net/20.500.11892/11457
dc.description.abstract Potential environmental hazards are posed by the presence of synthetic nitro aromatic compounds in soil and water. For this study nitro aromatic contaminants were divided into two groups: biologically orientated nitro aromatic compounds and industrial nitro aromatic compounds. As a model compound for biologically orientated nitro aromatic compounds, the biodegradation of atrazine was studied. Contamination of groundwater and soil by agricultural chemicals have become a growing concern worldwide. The entry of these chemicals into groundwater rivers and soil may have far-reaching consequences, i.e. for human and animal health. Accumulation may occur as a result of prescribed and improper use in agriculture, accidental spillage, sloppy clean-up of storage facilities, leaks at the dump sites and discharge of wastes from the production facilities. The need to develop methods for degradation and the removal of these chemicals from the environment therefore becomes essential. A variety of chemical and physical methods have been developed for this purpose, e.g. oxidation, reduction, hydrolysis, incineration, entrapment and burial. Most of the clean-up monitoring programs focus on the disappearance of the parent compound and in many cases little information is available on the degradation products. In this study the degradation potential of nitro aromatic agricultural chemicals by natural soil micro-organisms was evaluated and the potential for in situ degradation of these compounds was also evaluated in soil samples and in a laboratory soil column. The possibility of interaction between the soil and atrazine was investigated to compare the adsorption/desorption through a soil column. A variety of bacteria were isolated from various sources on atrazine containing minimal medium. The results demonstrated the ability of these organisms to degrade atrazine to an as yet unidentified metabolite. By using analitical methods such as MS/MS some of the daughter-ions of the metabolite were identified, however no final identification was made. The production and use of 2,4,6-trinitrotuelene (alpha TNT) is an integral component in the manufacture of munitions at ammunition plants. Significant quantities of waste are generated during the processes necessary for the production, purification and loading of munitions. As part of a bioremediation programme of contaminated areas, the potential for in situ degradation of these compounds was evaluated in soil samples and in a laboratory soil column. The significance of the interaction between the soil and some of these nitro aromatics was investigated to compare their mobility, adsorption/desorption through soil. A variety of bacteria from various samples was isolated on nitro aromatic containing minimal media. The ability of these bacteria to degrade nitro aromatic compounds was evaluated in pure culture and in natural consortia under both aerobic and anaerobic conditions. The consortia consisted mainly of Gram negative aerobic rods. The degree of degradation was determined by monitoring the presence of known intermediates by HPLC and MS/MS. The results obtained from the degradation of this compound under aerobic as well as anaerobic conditions clearly confirm the potential for in situ bioremediation of nitro aromatic contaminated soil. en
dc.language Afrikaans en
dc.subject Microbiology en
dc.title Biodegradering van sintetiese nitro-aromatiese verbindings deur mikro-organismes en
dc.type Masters degree en
dc.description.degree MSc en


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