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Towards molecular biological characterisation of the genes for sterol and bile acid metabolism in nocardioform bacteria

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dc.contributor.author Downing KJ en
dc.date.accessioned 2016-09-22T11:27:47Z
dc.date.available 2016-09-22T11:27:47Z
dc.date.submitted 1989 en
dc.identifier.uri http://hdl.handle.net/20.500.11892/123397
dc.description.abstract The steroids are a diversified class of oxygenated tetracyclic isoprenoid derivatives characterized by a cyclopentanoperhydrophenonthrene ring system. Two important groups of steroids are the sterols, of which cholesterol is the most important, and bile acids, which are converted from cholesterol. Commercial biotechnology operations dealing with steroids, centre around the use of microorganisms for transformations of steroid substrates into useful intermediates and final products. One group of bacteria capable of the before mentioned are the nocardiform bacteria. Cloning the genes of these organisms for the interconvertion of steroids and bile acids to pharmacologically important intermediates by complementation of the appropriate mutation was the main interest of this work. Mutagenesis using U.V.; NTG and EMS as mutagens, and the modified penicillin selection technique, resulted in several mutants, notably KD 1, which had lost its ability to utilize several carbon sources. The identification and determination of the suitability of these carbon sources was performed. Conditions of transformation of this mutant were optimized. The availability of a suitable vector led to the partial construction of a library of nocardioform chromosomal DNA in E.coll to clone the gene/genes of interest. en
dc.language English en
dc.title Towards molecular biological characterisation of the genes for sterol and bile acid metabolism in nocardioform bacteria en
dc.type Masters degree en
dc.description.degree MSc en


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