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'n Farmakochemiese ondersoek van gladiolus dalenii (Iridaceae)

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dc.contributor.advisor Gerritsma LM, Dr en
dc.contributor.author Botha S en
dc.date.accessioned 2016-09-22T07:18:16Z
dc.date.available 2016-09-22T07:18:16Z
dc.date.submitted 1987 en
dc.identifier.uri http://hdl.handle.net/20.500.11892/12574
dc.description.abstract Gladiolus dalenii van Geel is a species of Iridaceae better known as "swaardlelie" or Natal lily. This plant is indigenous to South Africa and can also be found in many gardens owing to the beauty of its flowers. Ethnomedicinal uses include oral dosing with corms to combat dysentery. <br><br> Leaves and corms of G. dalenii were investigated to determine the biological activity in a few test systems and also the metabolite composition of the different plant parts. The investigation was performed on plants gathered during a specific phase of the plant's development. Abnormal behaviour (Hippocratic evaluation) and postmortem organ lesions (macro-and microscopic) were noted as signs of acute toxicity in rats after intraperitoneal and oral administrations of plant suspensions. A primary test to determine bactericidal and fungicidal activity was performed using prokaryotic and eukaryotic test organisms. Determination of phototoxicity was done by using Candida albicans as a test organism. The presence of metabolites belonging to four major classes (polycyclic terpenoids, phenolic substances, substances containing nitrogen and carbohydrates) were determined by using primary chemical tests, thin-layer chromatography and microscopy. <br><br> It was established that leaves and corms of G. dalenii contain metabolites that exert toxic responses in rats. These metabolites affect mitotic active tissue (lymphoid tissue, the alimentary canal and testis) cardiac tissue (indirectly or directly), the liver and kidneys. Corm suspensions via intraperitoneal route are 10 times more toxic than leaves given via the same route and a 100 times more toxic than corm suspensions given orally. The metabolites responsible for toxicity seem to be saponins, as a highly toxic mixture of three (GDKE2) was isolated from corms. It can be concluded that these substances contribute to the frothing characteristics and hemolytic properties of G. dalenii. Leaves have a hemolytic index of 306, corms of 1834 and GDKE2 of 107390. Leaves contain at least one saponin and corms at least three. In addition to saponins phenolic substances, flavonoids, amino acids, sugars and calcium oxalate crystals were demonstrated in leaves and corms while starch was present in corms only. The plant material showed no bactericidal, fungicidal or phototoxic activity. <br><br> en
dc.language Afrikaans en
dc.subject Medical sciences: Pharmacology and therapeutics en
dc.title 'n Farmakochemiese ondersoek van gladiolus dalenii (Iridaceae) en
dc.type Masters degree en
dc.description.degree MSc en


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