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Saccharomyces cerevisiae engineered for xylan utilisation

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dc.contributor.advisor La Grange, D.C. Dr
dc.contributor.advisor Rose, S.H. Dr
dc.contributor.advisor Van Zyl, W.H. Prof
dc.contributor.author Mert, Marlin John
dc.date.accessioned 2018-02-09T13:38:03Z
dc.date.available 2018-02-09T13:38:03Z
dc.date.issued 2016
dc.identifier.uri http://hdl.handle.net/20.500.11892/182391
dc.description.abstract Bioethanal from plant biomass (lignocellulose) is an attractive alter - native to fossil fuels and does not compete with human food supplies. Xylan, made up of xylose units, represents a large component of lignocellulose. A Baker’s yeast strain was constructed that expresses enzymes required for xylan utilisation. To better understand the utilisation of xylan, the underlying principles of recombinant xylose metabolism was investigated using a metabolomics approach. The central carbon metabolism revealed that transaldolase activity in the pentose phosphate pathway is rate-limiting. In addition, the flux through glucose 1-phosphate and pyruvate in glycolysis limit the rate of xylose metabolism. en_ZA
dc.language.iso en en_ZA
dc.subject Saccharomyces en_ZA
dc.subject Saccharomyces cerevisiae engineering en_ZA
dc.subject Xylan - Utilisation en_ZA
dc.title Saccharomyces cerevisiae engineered for xylan utilisation en_ZA
dc.type Thesis en_ZA

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