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Remobilization of sucrose from the culm during germination of sugarcane setts

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dc.contributor.advisor Botha FC, Prof en
dc.contributor.advisor Groenewald H, Mr en
dc.contributor.author Boussiengui-Boussiengui G en
dc.date.accessioned 2016-09-22T08:35:26Z
dc.date.available 2016-09-22T08:35:26Z
dc.date.submitted 2005 en
dc.identifier.uri http://hdl.handle.net/20.500.11892/32231
dc.description.abstract The main substrate use during, shoot establishment from the lateral bud of sugarcane setts and enzymes involved in sucrose metabolism were investigated in the shoots and the internodes acting as source of carbohydrates. Radiolabelling studies were conducted to investigate the metabolism of sucrose and glucose during shoot establishment. The intemode's total dry mass over the 21-day of shoot establishment period was reduced by 25% and 30% in plants incubated in dark/light and total darkness, respectively. Sucrose accounted for 90% of the remobilized internodes' dry mass. From the remobilized sucrose 35%o and 50% were lost through respiration in plant incubated in dark/light and total darkness, respectively. All enzymes involved in sucrose cleavage/hydrolysis were induced in both the internodal tissues and the shoots. Northern analysis demonstrated that SAI transcripts were only detected in the internodal tissues after 14 days of shooting period when the sucrose content was reduced by one third compared to the day of planting. In contrast, CWI transcripts were observed throughout the shooting period in both tissue types. The highest CWI transcript levels were found after seven days in shoots and correlated with high hexose concentrations. The data suggests that both, SAI and CWI are expressed at transcript and protein level during shoot establishment. In both the internodes and the shoots, a cycle of breakdown and synthesis of sucrose was evident; despite the short labelling periods of one and three hours. From the data, it is evident that sucrose is the main form in which reduced carbon is translocated from the internodes (source) to the shoots (sink). Furthermore the data suggest that sucrose hydrolysis in the storage tissues is not required for export to the shoots. en
dc.language English en
dc.title Remobilization of sucrose from the culm during germination of sugarcane setts en
dc.type Masters degree en
dc.description.degree MSc en


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