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Purification and characterisation of the Amp C [beta]-lactamase from a South African Klebsiella pneumoniae isolate

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dc.contributor.advisor Essack SY, Prof en
dc.contributor.advisor Macdonald D, Mrs en
dc.contributor.author Govinden U en
dc.date.accessioned 2016-09-22T06:30:45Z
dc.date.available 2016-09-22T06:30:45Z
dc.date.submitted 2002 en
dc.identifier.uri http://hdl.handle.net/20.500.11892/4962
dc.description.abstract Bacterial resistance to antibiotics is a worldwide problem. This study purified and characterised the Amp C [beta]-lactamase from a South African Klebsiella pneumoniae isolate. The isolate's susceptibility to different classes of antibiotics was evaluated and [beta]-lactamase inducibility was confirmed using disc diffusion tests. The Amp C [beta]-lactamase was isolated on a phenylboronic acid agarose column, and further purified by dialysis and ultrafiltration before kinetic and inhibition parameters were determined. It was concluded that the inducible enzyme shows closest homology to the FOX-3 Amp C [beta]-lactamase but is possibly related to the FOX-1, MOX-1, CMY-1 Amp C [beta]-lactamase groups as a result of its resistance to cephamycins and not to carbapenems. en
dc.language English en
dc.subject Biotechnology en
dc.title Purification and characterisation of the Amp C [beta]-lactamase from a South African Klebsiella pneumoniae isolate en
dc.type Masters degree en
dc.description.degree MTech (Biotechnology) en


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